Review



ptppuc3  (Addgene inc)


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    Structured Review

    Addgene inc ptppuc3
    ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid <t>(pTpPuc3)</t> compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.
    Ptppuc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptppuc3/product/Addgene inc
    Average 85 stars, based on 1 article reviews
    ptppuc3 - by Bioz Stars, 2026-04
    85/100 stars

    Images

    1) Product Images from "Designer diatom episomes delivered by bacterial conjugation"

    Article Title: Designer diatom episomes delivered by bacterial conjugation

    Journal: Nature Communications

    doi: 10.1038/ncomms7925

    ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.
    Figure Legend Snippet: ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.

    Techniques Used: Conjugation Assay, Plasmid Preparation, Control, Expressing, Derivative Assay



    Similar Products

    ptppuc3  (Addgene inc)
    85
    Addgene inc ptppuc3
    ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid <t>(pTpPuc3)</t> compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.
    Ptppuc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptppuc3/product/Addgene inc
    Average 85 stars, based on 1 article reviews
    ptppuc3 - by Bioz Stars, 2026-04
    85/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.

    Journal: Nature Communications

    Article Title: Designer diatom episomes delivered by bacterial conjugation

    doi: 10.1038/ncomms7925

    Figure Lengend Snippet: ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.

    Article Snippet: E. coli strains containing plasmids p0521s (GenBank accession KP745602), pPtPuc3 (GenBank accession KP745601) and pTpPuc3 (GenBank accession KP745603) described in this manuscript have been deposited at Addgene.com and are available for request.

    Techniques: Conjugation Assay, Plasmid Preparation, Control, Expressing, Derivative Assay